• Immunofluorescence analysis of paraffin-embedded mouse brain using ELAVL3 Rabbit polyclonal antibody (STJ29931) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ELAVL3 in paraffin-embedded mouse brain using ELAVL3 Rabbit polyclonal antibody (STJ29931) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ELAVL3 in paraffin-embedded rat brain using ELAVL3 Rabbit polyclonal antibody (STJ29931) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using ELAVL3 Rabbit polyclonal antibody (STJ29931) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-ELAVL3 antibody (209-224) (STJ29931)

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STJ29931

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELAVL3
Gene ID: 1995
Uniprot ID: ELAV3_HUMAN
Immunogen Region: 209-224
Specificity: A synthetic peptide corresponding to a sequence within amino acids 161-260 of human ELAVL3 (NP_001411.2).
Immunogen Sequence: VTGVSRGVGFIRFDKRIEAE EAIKGLNGQKPLGAAEPITV KFANNPSQKTGQALLTHLYQ SSARRYAGPLHHQTQRFRLD NLLNMAYGVKSPLSLIARFS PIAIDGMSGLAGVGLSGGAA GAGWCIFVYNLSPEADESVL WQLFGPFGAVTNVKVIRDFT TNKCKGFGFVTMTNYDEAAM AIASLNGYRLGERVLQVSFK TSKQHKA
Tissue Specificity Brain specific.
Function RNA-binding protein that binds to AU-rich element (ARE) sequences of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance. Plays a role in the stabilization of GAP43 mRNA and in spatial learning.
Protein Name Elav-Like Protein 3
Hu-Antigen C
Huc
Paraneoplastic Cerebellar Degeneration-Associated Antigen
Paraneoplastic Limbic Encephalitis Antigen 21
Alternative Antibody Names Anti-Elav-Like Protein 3 antibody
Anti-Hu-Antigen C antibody
Anti-Huc antibody
Anti-Paraneoplastic Cerebellar Degeneration-Associated Antigen antibody
Anti-Paraneoplastic Limbic Encephalitis Antigen 21 antibody
Anti-ELAVL3 antibody
Anti-HUC antibody
Anti-PLE21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance