• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g HuR/ELAVL1 antibody (STJ11101733). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (STJ11101733) at a dilution of 1:1000.
  • Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human tonsil using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human kidney using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit monoclonal antibody (STJ11101733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ELAVL1 antibody (1-100) [S3MR] (STJ11101733)

SKU:
STJ11101733

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HuR/ELAVL1 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELAVL1
Gene ID: 1994
Uniprot ID: ELAV1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717).
Immunogen Sequence: MSNGYEDHMAEDCRGDIGRT NLIVNYLPQNMTQDELRSLF SSIGEVESAKLIRDKVAGHS LGYGFVNYVTAKDAERAINT LNGLRLQSKTIKVSYARPSS
Tissue Specificity Ubiquitous. Detected in brain, liver, thymus and muscle.
Post Translational Modifications Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD. Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
Function RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation. Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUUAGUUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.
Protein Name Elav-Like Protein 1
Hu-Antigen R
Hur
Database Links Reactome: R-HSA-450520
Cellular Localisation Cytoplasm
Nucleus
Stress Granule
P-Body
Translocates Into The Cytoplasm Following Phosphorylation By Mapkapk2
Likewise
Phosphorylation By Prkcd Promotes Translocation From The Nucleus Into The Cytoplasm
Where It Is Associated With Free And Cytoskeleton-Bound Polysomes
Localizes To The Stress Granules In The Presence Of Plekhn1
Alternative Antibody Names Anti-Elav-Like Protein 1 antibody
Anti-Hu-Antigen R antibody
Anti-Hur antibody
Anti-ELAVL1 antibody
Anti-HUR antibody

Information sourced from Uniprot.org

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