• Western blot analysis of lysates from Jurkat, HepG2, and A549 cells, using EIF5B Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-EIF5B antibody (1051-1100 aa) (STJ92886)

SKU:
STJ92886

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Eukaryotic translation initiation factor 5B (1051-1100 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF5B
Gene ID: 9669
Uniprot ID: IF2P_HUMAN
Immunogen Region: 1051-1100 aa
Specificity: eIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EIF5B at the amino acid range 1051-1100
Function Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit.
Protein Name Eukaryotic Translation Initiation Factor 5b
Eif-5b
Translation Initiation Factor If-2
Database Links Reactome: R-HSA-72706
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 5b antibody
Anti-Eif-5b antibody
Anti-Translation Initiation Factor If-2 antibody
Anti-EIF5B antibody
Anti-IF2 antibody
Anti-KIAA0741 antibody

Information sourced from Uniprot.org

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