| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF4E2 (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF4E2 |
| Gene ID: |
9470 |
| Uniprot ID: |
IF4E2_HUMAN |
| Immunogen Region: |
1-240 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF4E2 (NP_004837.1). |
| Immunogen Sequence: |
MNNKFDALKDDDSGDHDQNE ENSTQKDGEKEKTERDKNQS SSKRKAVVPGPAEHPLQYNY TFWYSRRTPGRPTSSQSYEQ NIKQIGTFASVEQFWRFYSH MVRPGDLTGHSDFHLFKEGI KPMWEDDANKNGGKWIIRLR KGLASRCWENLILAMLGEQF MVGEEICGAVVSVRFQEDII SIWNKTASDQATTARIRDTL RRVLNLPPNTIMEYKTHTDS IKMPGRLGPQRLLFQNLWK |
| Post Translational Modifications | Ubiquitinated by ARIH1. The consequences of ubiquitination are however unclear: according to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report ubiquitination leads to its subsequent degradation. ISGylation enhances its cap structure-binding activity and translation-inhibition activity. |
| Function | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. In P-bodies, component of a complex that promotes miRNA-mediated translational repression. Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism. Component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation. In association with GIGYF2, assists ribosome-associated quality control (RQC) by sequestering the mRNA cap, blocking ribosome initiation and decreasing the translational load on problematic messages. Part of a pathway that works in parallel to RQC-mediated degradation of the stalled nascent polypeptide. GIGYF2 and EIF4E2 work downstream and independently of ZNF598, which seems to work as a scaffold that can recruit them to faulty mRNA even if alternative recruitment mechanisms may exist. (Microbial infection) Upon SARS coronavirus-2/SARS-CoV-2 infection, the interaction with non-structural protein 2 (nsp2) with GIGYF2 enhances GIGYF2 binding to EIF4E2 and increases repression of translation initiation of genes involved in antiviral innate immune response such as IFNB1. |
| Protein Name | Eukaryotic Translation Initiation Factor 4e Type 2Eif-4e Type 2Eif4e Type 2Eukaryotic Translation Initiation Factor 4e Homologous ProteinEukaryotic Translation Initiation Factor 4e-Like 3Eif4e-Like Protein 4e-LpMrna Cap-Binding Protein 4ehpH4ehpMrna Cap-Binding Protein Type 3 |
| Database Links | Reactome: R-HSA-1169408 |
| Cellular Localisation | CytoplasmP-Body |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4e Type 2 antibodyAnti-Eif-4e Type 2 antibodyAnti-Eif4e Type 2 antibodyAnti-Eukaryotic Translation Initiation Factor 4e Homologous Protein antibodyAnti-Eukaryotic Translation Initiation Factor 4e-Like 3 antibodyAnti-Eif4e-Like Protein 4e-Lp antibodyAnti-Mrna Cap-Binding Protein 4ehp antibodyAnti-H4ehp antibodyAnti-Mrna Cap-Binding Protein Type 3 antibodyAnti-EIF4E2 antibodyAnti-EIF4EL3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
