• Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301). Western blot was performed from the immunoprecipitate using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) antibody ( STJ112301) at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using eIF2B epsilon (eIF2B epsilon (EIF2B5) ) Rabbit polyclonal antibody (STJ112301) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) Rabbit polyclonal antibody (STJ112301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B5 antibody (442-721) (STJ112301)

SKU:
STJ112301

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B5
Gene ID: 8893
Uniprot ID: EI2BE_HUMAN
Immunogen Region: 442-721
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human eIF2B epsilon (eIF2B epsilon (EIF2B5) ) (NP_003898.2).
Immunogen Sequence: TLPEGSVISLHPPDAEEDED DGEFSDDSGADQEKDKVKMK GYNPAEVGAAGKGYLWKAAG MNMEEEEELQQNLWGLKINM EEESESESEQSMDSEEPDSR GGSPQMDDIKVFQNEVLGTL QRGKEENISCDNLVLEINSL KYAYNISLKEVMQVLSHVVL EFPLQQMDSPLDSSRYCALL LPLLKAWSPVFRNYIKRAAD HLEALAAIEDFFLEHEALGI SMAKVLMAFYQLEILAEET
Post Translational Modifications Phosphorylated at Ser-544 by DYRK2.this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B.phosphorylation inhibits its function. Polyubiquitinated, probably by NEDD4.
Function Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.
Protein Name Translation Initiation Factor Eif2b Subunit Epsilon
Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon
Database Links Reactome: R-HSA-72731
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Translation Initiation Factor Eif2b Subunit Epsilon antibody
Anti-Eif2b Gdp-Gtp Exchange Factor Subunit Epsilon antibody
Anti-EIF2B5 antibody
Anti-EIF2BE antibody

Information sourced from Uniprot.org

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