• Immunofluorescence analysis of U2OS cells using EIF2B4 Rabbit polyclonal antibody (STJ11100161) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using EIF2B4 Rabbit polyclonal antibody (STJ11100161) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using EIF2B4 Rabbit polyclonal antibody (STJ11100161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B4 antibody (30-100) (STJ11100161)

SKU:
STJ11100161

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF2B4 (30-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B4
Gene ID: 8890
Uniprot ID: EI2BD_HUMAN
Immunogen Region: 30-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human EIF2B4 (Q9UI10).
Immunogen Sequence: EMTKEEKLQLRKEKKQQKKK RKEEKGAEPETGSAVSAAQC QVGPTRELPESGIQLGTPRE KVPAGRSKAEL
Function Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. (Microbial infection) In case of infection by influenza A virus.required for viral replication in tracheal cells.
Protein Name Translation Initiation Factor Eif-2b Subunit Delta
Eif-2b Gdp-Gtp Exchange Factor Subunit Delta
Database Links Reactome: R-HSA-72731
Alternative Antibody Names Anti-Translation Initiation Factor Eif-2b Subunit Delta antibody
Anti-Eif-2b Gdp-Gtp Exchange Factor Subunit Delta antibody
Anti-EIF2B4 antibody
Anti-EIF2BD antibody

Information sourced from Uniprot.org

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