• Western blot analysis of extracts of various cell lines, using eIF2A rabbit monoclonal antibody (STJ11102530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of Rat lung, using eIF2A rabbit monoclonal antibody (STJ11102530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreas using eIF2A rabbit monoclonal antibody (STJ11102530) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using eIF2A rabbit monoclonal antibody (STJ11102530) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of eIF2A in paraffin-embedded human tonsil tissue using eIF2A rabbit monoclonal antibody (STJ11102530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of eIF2A in paraffin-embedded rat brain tissue using eIF2A rabbit monoclonal antibody (STJ11102530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of eIF2A in paraffin-embedded rat liver tissue using eIF2A rabbit monoclonal antibody (STJ11102530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-EIF2A antibody (450-550) [S0MR] (STJ11102530)

SKU:
STJ11102530

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-eIF2A (450-550) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2A
Gene ID: 83939
Uniprot ID: EIF2A_HUMAN
Immunogen Region: 450-550
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 450-550 of human eIF2A (Q9BY44).
Immunogen Sequence: ALRNKPITNSKLHEEEPPQN MKPQSGNDKPLSKTALKNQR KHEAKKAAKQEARSDKSPDL APTPAPQSTPRNTVSQSISG DPEIDKKIKNLKKKLKAIEQ L
Tissue Specificity Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
Function Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
Protein Name Eukaryotic Translation Initiation Factor 2a
Eif-2a
65 Kda Eukaryotic Translation Initiation Factor 2a Cleaved Into - Eukaryotic Translation Initiation Factor 2a - N-Terminally Processed
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2a antibody
Anti-Eif-2a antibody
Anti-65 Kda Eukaryotic Translation Initiation Factor 2a Cleaved Into - Eukaryotic Translation Initiation Factor 2a - N-Terminally Processed antibody
Anti-EIF2A antibody
Anti-CDA02 antibody
Anti-MSTP004 antibody
Anti-MSTP089 antibody

Information sourced from Uniprot.org

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