• Western blot analysis of extracts of A375 cells, using EHMT2 antibody (STJ11103018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded human breast tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded human esophagus tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded human thyroid tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded mouse colon tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded mouse lung tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded rat kidney tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EHMT2 in paraffin-embedded rat spleen tissue using EHMT2 rabbit monoclonal antibody (STJ11103018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-EHMT2 antibody (2-300) [S8MR] (STJ11103018)

SKU:
STJ11103018

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-EHMT2 (2-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHMT2
Gene ID: 10919
Uniprot ID: EHMT2_HUMAN
Immunogen Region: 2-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-300 of human EHMT2 (Q96KQ7).
Immunogen Sequence: AAAAGAAAAAAAEGEAPAEM GALLLEKETRGATERVHGSL GDTPRSEETLPKATPDSLEP AGPSSPASVTVTVGDEGADT PVGATPLIGDESENLEGDGD LRGGRILLGHATKSFPSSPS KGGSCPSRAKMSMTGAGKSP PSVQSLAMRLLSMPGAQGAA AAGSEPPPATTSPEGQPKVH RARKTMSKPGNGQPPVPEKR PPEIQHFRMSDDVHSLGKVT SDLAKRRKLNSGGGLSEEL
Tissue Specificity Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
Post Translational Modifications Methylated at Lys-185.automethylated.
Function Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. Recruited to the promoters of target genes through interaction with transcriptional repressor MSX1, leading to the inhibition of myoblast differentiation via transcriptional repression of differentiation factors.
Protein Name Histone-Lysine N-Methyltransferase Ehmt2
Euchromatic Histone-Lysine N-Methyltransferase 2
Hla-B-Associated Transcript 8
Histone H3-K9 Methyltransferase 3
H3-K9-Hmtase 3
Lysine N-Methyltransferase 1c
Protein G9a
Database Links Reactome: R-HSA-2559582
Reactome: R-HSA-3214841
Reactome: R-HSA-427389
Reactome: R-HSA-6804760
Reactome: R-HSA-73762
Reactome: R-HSA-8853884
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Chromosome
Associates With Euchromatic Regions
Does Not Associate With Heterochromatin
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Ehmt2 antibody
Anti-Euchromatic Histone-Lysine N-Methyltransferase 2 antibody
Anti-Hla-B-Associated Transcript 8 antibody
Anti-Histone H3-K9 Methyltransferase 3 antibody
Anti-H3-K9-Hmtase 3 antibody
Anti-Lysine N-Methyltransferase 1c antibody
Anti-Protein G9a antibody
Anti-EHMT2 antibody
Anti-BAT8 antibody
Anti-C6orf30 antibody
Anti-G9A antibody
Anti-KMT1C antibody
Anti-NG36 antibody

Information sourced from Uniprot.org

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