• Western blot analysis of various lysates using EHD4 Rabbit polyclonal antibody (STJ115713) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EHD4 antibody (362-541) (STJ115713)

SKU:
STJ115713

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EHD4 (362-541) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHD4
Gene ID: 30844
Uniprot ID: EHD4_HUMAN
Immunogen Region: 362-541
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 362-541 of human EHD4 (NP_644670.1).
Immunogen Sequence: MQEQLENYDFTKFHSLKPKL IEAVDNMLSNKISPLMNLIS QEETSTPTQLVQGGAFDGTT EGPFNQGYGEGAKEGADEEE WVVAKDKPVYDELFYTLSPI NGKISGVNAKKEMVTSKLPN SVLGKIWKLADCDCDGMLDE EEFALAKHLIKIKLDGYELP SSLPPHLVPPSHRKSLPKAD
Tissue Specificity Highly expressed in pancreas and heart.
Function ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
Protein Name Eh Domain-Containing Protein 4
Hepatocellular Carcinoma-Associated Protein 10/11
Past Homolog 4
Cellular Localisation Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Recycling Endosome Membrane
Cell Membrane
Alternative Antibody Names Anti-Eh Domain-Containing Protein 4 antibody
Anti-Hepatocellular Carcinoma-Associated Protein 10/11 antibody
Anti-Past Homolog 4 antibody
Anti-EHD4 antibody
Anti-HCA10 antibody
Anti-HCA11 antibody
Anti-PAST4 antibody
Anti-FKSG7 antibody

Information sourced from Uniprot.org

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