• STJ70596 (0. 1µg/ml) staining of A549 (A) and (0. 3µg/ml) HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70596 (0. 3µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70596 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70596 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70596 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-EHD2 antibody (C-Term) (STJ70596)

SKU:
STJ70596-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EHD2 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
IF-Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EHD2
Gene ID: 30846
Uniprot ID: EHD2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_055416.2
Specificity: This antibody is expected to recognise EHD1 protein as well as EHD2.
Immunogen Sequence: CRLVPPSKRRHKGSA
Function ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane. Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane.
Protein Name Eh Domain-Containing Protein 2
Past Homolog 2
Database Links Reactome: R-HSA-983231
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Caveola
Endosome Membrane
Cytoplasm
Cytosol
Colocalizes With Glut4 In Intracellular Tubulovesicular Structures That Are Associated With Cortical F-Actin
Colocalizes With Fer1l5 At Plasma Membrane In Myoblasts And Myotubes
Alternative Antibody Names Anti-Eh Domain-Containing Protein 2 antibody
Anti-Past Homolog 2 antibody
Anti-EHD2 antibody
Anti-PAST2 antibody

Information sourced from Uniprot.org

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