• Western blot analysis of extracts of various cell lines, using EFHC1 antibody (STJ110309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using EFHC1 antibody (STJ110309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EFHC1 antibody (391-640) (STJ110309)

SKU:
STJ110309

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EFHC1
Gene ID: 114327
Uniprot ID: EFHC1_HUMAN
Immunogen Region: 391-640
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 391-640 of human EFHC1 (NP_060570.2).
Immunogen Sequence: EDSAQNCFALIPKAPKKDVI KMLVNDNKVLRYLAVLESPI PEDKDRRFVFSYFLATDMIS IFEPPVRNSGIIGGKYLGRT KVVKPYSTVDNPVYYGPSDF FIGAVIEVFGHRFIILDTDE YVLKYMESNAAQYSPEALAS IQNHVRKREAPAPEAESKQT EKDPGVQELEALIDTIQKQL KDHSCKDNIREAFQIYDKEA SGYVDRDMFFKICESLNVPV DDSLVKELIRMCSHGEGKI
Tissue Specificity Widely expressed. Not detected in lymphocytes.
Function Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Microtubule-associated protein which regulates cell division and neuronal migration during cortical development. Necessary for radial and tangential cell migration during brain development, possibly acting as a regulator of cell morphology and process formation during migration. May enhance calcium influx through CACNA1E and stimulate programmed cell death.
Protein Name Ef-Hand Domain-Containing Protein 1
Myoclonin-1
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Axoneme
Flagellum Axoneme
Microtubule Organizing Center
Centrosome
Spindle
Spindle Pole
Alternative Antibody Names Anti-Ef-Hand Domain-Containing Protein 1 antibody
Anti-Myoclonin-1 antibody
Anti-EFHC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance