| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EEFSEC |
| Gene ID: |
60678 |
| Uniprot ID: |
SELB_HUMAN |
| Immunogen Region: |
347-596 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 347-596 of human EEFSEC (NP_068756.2). |
| Immunogen Sequence: |
RLMFFSPAPDNFDQEPILDS FNFSQEYLFQEQYLSKDLTP AVTDNDEADKKAGQATEGHC PRQQWALVEFEKPVTCPRLC LVIGSRLDADIHTNTCRLAF HGILLHGLEDRNYADSFLPR LKVYKLKHKHGLVERAMDDY SVIGRSLFKKETNIQLFVGL KVHLSTGELGIIDSAFGQSG KFKIHIPGGLSPESKKILTP ALKKRARAGRGEATRQEESA ERSEPSQHVVLSLTFKRYV |
| Function | Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons. Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. |
| Protein Name | Selenocysteine-Specific Elongation FactorElongation Factor SecEukaryotic Elongation Factor - Selenocysteine-Trna-Specific |
| Database Links | Reactome: R-HSA-2408557 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Selenocysteine-Specific Elongation Factor antibodyAnti-Elongation Factor Sec antibodyAnti-Eukaryotic Elongation Factor - Selenocysteine-Trna-Specific antibodyAnti-EEFSEC antibodyAnti-SELB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
