| Short Description : | Rabbit polyclonal Endothelin 1 (18-212) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EDN1 |
| Gene ID: | 1906 |
| Uniprot ID: | EDN1_HUMAN |
| Immunogen Region: | 18-212 |
| Immunogen Sequence: | APETAVLGAELSAVGENGGE KPTPSPPWRLRRSKRCSCSS LMDKECVYFCHLDIIWVNTP EHVVPYGLGSPRSKRALENL LPTKATDRENRCQCASQKDK KCWNFCQAGKELRAEDIMEK DWNNHKKGKDCSKLGKKCIY QQLVRGRKIRRSSEEHLRQT RSETMRNSVKSSFHDPKLKG KPSRERYVTHNRAHW |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human Endothelin 1 (NP_001946.3). |
| Tissue Specificity | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2. |
| Protein Name | Endothelin-1Preproendothelin-1Ppet1 Cleaved Into - Endothelin-1Et-1 - Big Endothelin-1 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-9856649 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Endothelin-1 antibodyAnti-Preproendothelin-1 antibodyAnti-Ppet1 Cleaved Into - Endothelin-1 antibodyAnti-Et-1 - Big Endothelin-1 antibodyAnti-EDN1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit polyclonal antibody (STJ119977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98762/382815/STJ119977_1__41595.1713151309.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit polyclonal antibody (STJ119977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
