• Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit polyclonal antibody (STJ119977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-EDN1 antibody (18-212) (STJ119977)

SKU:
STJ119977

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDN1 (18-212) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDN1
Gene ID: 1906
Uniprot ID: EDN1_HUMAN
Immunogen Region: 18-212
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human EDN1 (NP_001946.3).
Immunogen Sequence: APETAVLGAELSAVGENGGE KPTPSPPWRLRRSKRCSCSS LMDKECVYFCHLDIIWVNTP EHVVPYGLGSPRSKRALENL LPTKATDRENRCQCASQKDK KCWNFCQAGKELRAEDIMEK DWNNHKKGKDCSKLGKKCIY QQLVRGRKIRRSSEEHLRQT RSETMRNSVKSSFHDPKLKG KPSRERYVTHNRAHW
Tissue Specificity Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Function Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor.
Protein Name Endothelin-1
Preproendothelin-1
Ppet1 Cleaved Into - Endothelin-1
Et-1 - Big Endothelin-1
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-416476
Cellular Localisation Secreted
Alternative Antibody Names Anti-Endothelin-1 antibody
Anti-Preproendothelin-1 antibody
Anti-Ppet1 Cleaved Into - Endothelin-1 antibody
Anti-Et-1 - Big Endothelin-1 antibody
Anti-EDN1 antibody

Information sourced from Uniprot.org

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