| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-EDF1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1µg/ml.IHC: In paraffin embedded Human |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | EDF1 |
| Gene ID: | 8721 |
| Uniprot ID: | EDF1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_003783.1; NP_694880.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1). |
| Immunogen Sequence: | NKQHSITKNTAKLDR |
| Post Translational Modifications | Phosphorylated (by PKA and PKC). |
| Function | Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. |
| Protein Name | Endothelial Differentiation-Related Factor 1Edf-1Multiprotein-Bridging Factor 1Mbf1 |
| Cellular Localisation | CytoplasmNucleusAlso Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin |
| Alternative Antibody Names | Anti-Endothelial Differentiation-Related Factor 1 antibodyAnti-Edf-1 antibodyAnti-Multiprotein-Bridging Factor 1 antibodyAnti-Mbf1 antibodyAnti-EDF1 antibody |
Information sourced from Uniprot.org