| Tissue Specificity | Expressed in brain, liver, kidney and heart (at protein level). Also expressed in testis. |
| Post Translational Modifications | Phosphorylated. |
| Function | Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. |
| Protein Name | Endothelial Differentiation-Related Factor 1Edf-1Multiprotein-Bridging Factor 1Mbf1 |
| Cellular Localisation | CytoplasmNucleusAlso Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin |
| Alternative Antibody Names | Anti-Endothelial Differentiation-Related Factor 1 antibodyAnti-Edf-1 antibodyAnti-Multiprotein-Bridging Factor 1 antibodyAnti-Mbf1 antibodyAnti-Edf1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/185810/407715/STJ11106614_1__42693.1744894238.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.")
![Western blot analysis of lysates from HT-29 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/185810/407716/STJ11106614_2__77292.1744894239.jpg?c=1 "Western blot analysis of lysates from HT-29 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.")
![Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/185810/407715/STJ11106614_1__42693.1744894238.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (STJ11106614) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.")
