• Western blot analysis of various lysates using ECHS1 rabbit polyclonal antibody (STJ111198) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Immunofluorescence analysis of HeLa cells using ECHS1 rabbit polyclonal antibody (STJ111198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using ECHS1 rabbit polyclonal antibody (STJ111198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ECHS1 rabbit polyclonal antibody (STJ111198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ECHS1 antibody (28-290) (STJ111198)

SKU:
STJ111198

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECHS1 (28-290) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECHS1
Gene ID: 1892
Uniprot ID: ECHM_HUMAN
Immunogen Region: 28-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-290 of human ECHS1 (NP_004083.3).
Immunogen Sequence: ASGANFEYIIAEKRGKNNTV GLIQLNRPKALNALCDGLID ELNQALKTFEEDPAVGAIVL TGGDKAFAAGADIKEMQNLS FQDCYSSKFLKHWDHLTQVK KPVIAAVNGYAFGGGCELAM MCDIIYAGEKAQFAQPEILI GTIPGAGGTQRLTRAVGKSL AMEMVLTGDRISAQDAKQAG LVSKICPVETLVEEAIQCAE KIASNSKIVVAMAKESVNAA FEMTLTEGSKLEKKLFYST
Tissue Specificity Liver, fibroblast, muscle. Barely detectable in spleen and kidney.
Function Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16. Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate. Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA).
Protein Name Enoyl-Coa Hydratase - Mitochondrial
Mech
Mech1
Enoyl-Coa Hydratase 1
Echs1
Short-Chain Enoyl-Coa Hydratase
Sceh
Database Links Reactome: R-HSA-70895
Reactome: R-HSA-77310
Reactome: R-HSA-77346
Reactome: R-HSA-77348
Reactome: R-HSA-77350
Reactome: R-HSA-77352
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Enoyl-Coa Hydratase - Mitochondrial antibody
Anti-Mech antibody
Anti-Mech1 antibody
Anti-Enoyl-Coa Hydratase 1 antibody
Anti-Echs1 antibody
Anti-Short-Chain Enoyl-Coa Hydratase antibody
Anti-Sceh antibody
Anti-ECHS1 antibody

Information sourced from Uniprot.org

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