• Western blot analysis of extracts of various cell lines, using ECE2 antibody (STJ29129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ECE2 antibody (1-160) (STJ29129)

SKU:
STJ29129

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-160
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ECE2 (NP_115707.2).
Immunogen Sequence: MASPGAGRAPPELPERNCGY REVEYWDQRYQGAADSAPYD WFGDFSSFRALLEPELRPED RILVLGCGNSALSYELFLGG FPNVTSVDYSSVVVAAMQAR HAHVPQLRWETMDVRKLDFP SASFDVVLEKGTLDALLAGE RDPWTVSSEGVHTVDQVLSE
Background Enables metalloendopeptidase activity. Involved in peptide hormone processing. Located in cytoplasmic vesicle membrane.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance