• Western blot analysis of extracts of various cell lines, using ECE2 antibody (STJ29129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ECE2 antibody (1-160) (STJ29129)

SKU:
STJ29129

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECE2 (1-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ECE2 (NP_115707.2).
Immunogen Sequence: MASPGAGRAPPELPERNCGY REVEYWDQRYQGAADSAPYD WFGDFSSFRALLEPELRPED RILVLGCGNSALSYELFLGG FPNVTSVDYSSVVVAAMQAR HAHVPQLRWETMDVRKLDFP SASFDVVLEKGTLDALLAGE RDPWTVSSEGVHTVDQVLSE
Background The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase may activate cyclin-dependent kinase 2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Information sourced from Uniprot.org

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