• Western blot analysis of 1) Hela Cell, 2) 293T Cell, 3) Mouse Brain Tissue, 4) Rat Brain Tissue lysate using SGT1/ECDRabbit polyclonal Antibody diluted at 1:2,000.
  • Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ECD antibody (236-286 aa) (STJ198327)

SKU:
STJ198327

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein ecdysoneless homolog (236-286 aa) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECD
Gene ID: 11319
Uniprot ID: ECD_HUMAN
Immunogen Region: 236-286 aa
Specificity: This antibody detects endogenous levels of SGT1/ECD at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the human SGT1/ECD at the amino acid range 236-286
Post Translational Modifications Phosphorylated predominantly by CK2 on two serine-containing clusters.involved in cell cycle regulation activity.
Function Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
Protein Name Protein Ecdysoneless Homolog
Human Suppressor Of Gcr Two
Hsgt1
Cellular Localisation Cytoplasm
Nucleus
Predominantly Is Located In The Cytoplasm
Alternative Antibody Names Anti-Protein Ecdysoneless Homolog antibody
Anti-Human Suppressor Of Gcr Two antibody
Anti-Hsgt1 antibody
Anti-ECD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance