• Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit monoclonal antibody (STJ11103122, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of C6 cells using EB3/MAPRE3 Rabbit monoclonal antibody (STJ11103122, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using EB3/MAPRE3 Rabbit monoclonal antibody (STJ11103122, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using EB3/MAPRE3 Rabbit monoclonal antibody (STJ11103122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EB3/MAPRE3 antibody (182-281) [S2MR] (STJ11103122)

SKU:
STJ11103122

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPRE3
Gene ID: 22924
Uniprot ID: MARE3_HUMAN
Immunogen Region: 182-281
Specificity: A synthetic peptide corresponding to a sequence within amino acids 182-281 of human EB3/MAPRE3 (Q9UPY8).
Immunogen Sequence: CILRKNPPSARNGGHETDAQ ILELNQQLVDLKLTVDGLEK ERDFYFSKLRDIELICQEHE SENSPVISGIIGILYATEEG FAPPEDDEIEEHQQEDQDEY
Tissue Specificity Predominantly expressed in brain and muscle.
Function Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules.
Protein Name Microtubule-Associated Protein Rp/Eb Family Member 3
Eb1 Protein Family Member 3
Ebf3
End-Binding Protein 3
Eb3
Rp3
Cellular Localisation Cytoplasm
Cytoskeleton
Associated With The Microtubule Network
Detected At The Plus End Of Microtubules
Alternative Antibody Names Anti-Microtubule-Associated Protein Rp/Eb Family Member 3 antibody
Anti-Eb1 Protein Family Member 3 antibody
Anti-Ebf3 antibody
Anti-End-Binding Protein 3 antibody
Anti-Eb3 antibody
Anti-Rp3 antibody
Anti-MAPRE3 antibody

Information sourced from Uniprot.org

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