• CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g E2F6 rabbit monoclonal antibody (STJ11101356) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of E2F6 in representative gene loci (BRCA1) , as shown in figure.
  • Western blot analysis of extracts of various cell lines, using E2F6 rabbit monoclonal antibody (STJ11101356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g E2F6 antibody (STJ11101356). Western blot was performed from the immunoprecipitate using E2F6 antibody (STJ11101356) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of 293T cells, using E2F6 antibody (STJ11101356) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-E2F6 antibody (110-281) [S6MR] (STJ11101356)

SKU:
STJ11101356

$174.83 - $775.58
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Host: Rabbit
Applications: WB/IP/ELISA/ChIP/CUT&Tag
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
ChIP:5 Mu g anti
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F6
Gene ID: 1876
Uniprot ID: E2F6_HUMAN
Immunogen Region: 110-281
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 110-281 of human E2F6 (NP_937987.2)
Immunogen Sequence: LDGIDLVEKKSKNHIRWIGS DLSNFGAVPQQKKLQEELSD LSAMEDALDELIKDCAQQLF ELTDDKENERLAYVTYQDIH SIQAFHEQIVIAVKAPAETR LDVPAPREDSITVHIRSTNG PIDVYLCEVEQGQTSNKRSE GVGTSSSESTHPEGPEEEEN PQQSEELLEVSN
Tissue Specificity Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Function Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Represses expression of some meiosis-specific genes, including SLC25A31/ANT4. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Protein Name Transcription Factor E2f6
E2f-6
Database Links Reactome: R-HSA-69205
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f6 antibody
Anti-E2f-6 antibody
Anti-E2F6 antibody

Information sourced from Uniprot.org

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