• Western blot analysis of lysates from HepG2, K562, and COLO205 cells, using E2F2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using E2F-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F2 antibody (221-270 aa) (STJ92808)

SKU:
STJ92808

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription factor E2F2 (221-270 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F2
Gene ID: 1870
Uniprot ID: E2F2_HUMAN
Immunogen Region: 221-270 aa
Specificity: E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human E2F2 at the amino acid range 221-270
Post Translational Modifications Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Protein Name Transcription Factor E2f2
E2f-2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-69231
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f2 antibody
Anti-E2f-2 antibody
Anti-E2F2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance