• Western blot analysis of extracts of various cell lines, using DUSP15 antibody (STJ118752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using DUSP15 rabbit polyclonal antibody (STJ118752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DUSP15 antibody (10-90) (STJ118752)

SKU:
STJ118752

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DUSP15 (10-90) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP15
Gene ID: 128853
Uniprot ID: DUS15_HUMAN
Immunogen Region: 10-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-90 of human DUSP15 (NP_542178.2).
Immunogen Sequence: PGLYLGNFIDAKDLDQLGRN KITHIISIHESPQPLLQDIT YLRIPVADTPEVPIKKHFKE CINFIHCCRLNGGNCLVHCF A
Tissue Specificity Highly expressed in testis. Expressed in brain.up-regulated in patients with multiple sclerosis gray matter lesions.
Function May dephosphorylate MAPK13, ATF2, ERBB3, PDGFRB and SNX6. Isoform 3: May play a role in the regulation of oligodendrocyte differentiation. May play a role in the regulation of myelin formation. Involved in the regulation of Erk1/2 phosphorylation in Schwann cells.the signaling may be linked to the regulation of myelination.
Protein Name Dual Specificity Protein Phosphatase 15
Vh1-Related Member Y
Vaccinia Virus Vh1-Related Dual-Specific Protein Phosphatase Y
Cellular Localisation Cytoplasm
Isoform 3: Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 15 antibody
Anti-Vh1-Related Member Y antibody
Anti-Vaccinia Virus Vh1-Related Dual-Specific Protein Phosphatase Y antibody
Anti-DUSP15 antibody
Anti-C20orf57 antibody
Anti-VHY antibody

Information sourced from Uniprot.org

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