Anti-DSG2 antibody (635-935) (STJ11104316)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Desmoglein-2 (635-935) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DSG2 |
| Gene ID | 1829 |
| Uniprot ID | DSG2_HUMAN |
| Immunogen Region | 635-935 |
| Immunogen Sequence | CHCGKGAKGFTPIPGTIEML HPWNNEGAPPEDKVVPSFLP VDQGGSLVGRNGVGGMAKEA TMKGSSSASIVKGQHEMSEM DGRWEEHRSLLSGRATQFTG ATGAIMTTETTKTARATGAS RDMAGAQAAAVALNEEFLRN YFTDKAASYTEEDENHTAKD CLLVYSQEETESLNASIGCC SFIEGELDDRFLDDLGLKFK TLAEVCLGQKIDINKEIEQR QKPATETSMNTASHSLCEQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 635-935 of human DSG2 (NP_001934.2). |
Additional Info
| Tissue Specificity | Expressed in undifferentiated pluripotent stem cells, expression decreases during differentiation (at protein level). Expressed in hematopoietic stem cells and circulating endothelial progenitor cells, expression decreases upon increasing cell lineage commitment (at protein level). Expressed on common myeloid progenitors, pro-myelocytes, pro-erythrocytes and B-cell linage progenitors (at protein level). Expression in mature cell types in the bone marrow and mature leukocyte populations is absent. Expressed by foreskin fibroblasts, expression peaks during the early stage of differentiation reprogramming (at protein level). Expressed by endothelial cells in both arterioles and venules in the cervix (at protein level). Expressed in kidney tubular epithelial cells. |
| Post Translational Modifications | Palmitoylated by ZDHHC5 at the plasma membrane. |
| Function | A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Required for proliferation and viability of embryonic stem cells in the blastocyst, thereby crucial for progression of post-implantation embryonic development. Maintains pluripotency by regulating epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via interacting with and sequestering CTNNB1 to sites of cell-cell contact, thereby reducing translocation of CTNNB1 to the nucleus and subsequent transcription of CTNNB1/TCF-target genes. Promotes pluripotency and the multi-lineage differentiation potential of hematopoietic stem cells. Plays a role in endothelial cell sprouting and elongation via mediating the junctional-association of cortical actin fibers and CDH5. Plays a role in limiting inflammatory infiltration and the apoptotic response to injury in kidney tubular epithelial cells, potentially via its role in maintaining cell-cell adhesion and the epithelial barrier. |
| Protein Name | Desmoglein-2Cadherin Family Member 5Hdgc |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-9013423 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionDesmosomeCytoplasm |
| Alternative Antibody Names | Anti-Desmoglein-2 antibodyAnti-Cadherin Family Member 5 antibodyAnti-Hdgc antibodyAnti-DSG2 antibodyAnti-CDHF5 antibody |
Information sourced from Uniprot.org