| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Desmoglein-2 (635-935) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DSG2 |
| Gene ID: |
1829 |
| Uniprot ID: |
DSG2_HUMAN |
| Immunogen Region: |
635-935 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 635-935 of human DSG2 (NP_001934.2). |
| Immunogen Sequence: |
CHCGKGAKGFTPIPGTIEML HPWNNEGAPPEDKVVPSFLP VDQGGSLVGRNGVGGMAKEA TMKGSSSASIVKGQHEMSEM DGRWEEHRSLLSGRATQFTG ATGAIMTTETTKTARATGAS RDMAGAQAAAVALNEEFLRN YFTDKAASYTEEDENHTAKD CLLVYSQEETESLNASIGCC SFIEGELDDRFLDDLGLKFK TLAEVCLGQKIDINKEIEQR QKPATETSMNTASHSLCEQ |
| Tissue Specificity | All of the tissues tested and carcinomas. |
| Post Translational Modifications | Palmitoylated by ZDHHC5 at the plasma membrane. |
| Function | Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. |
| Protein Name | Desmoglein-2Cadherin Family Member 5Hdgc |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-6805567Reactome: R-HSA-6809371Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-9013423 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionDesmosome |
| Alternative Antibody Names | Anti-Desmoglein-2 antibodyAnti-Cadherin Family Member 5 antibodyAnti-Hdgc antibodyAnti-DSG2 antibodyAnti-CDHF5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DSG2 knockout (KO) A-549 cells, using [KO Validated] DSG2 Rabbit polyclonal antibody (STJ11100872) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89806/358273/STJ11100872_1__45236.1713122617.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DSG2 knockout (KO) A-549 cells, using [KO Validated] DSG2 Rabbit polyclonal antibody (STJ11100872) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
