• Western blot analysis of extracts of various cell lines, using Dopamine Receptor D3 (DRD3) Rabbit monoclonal antibody (STJ11102110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DRD3 antibody (1-100) [S0MR] (STJ11102110)

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STJ11102110

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Dopamine Receptor D3 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DRD3
Gene ID: 1814
Uniprot ID: DRD3_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Dopamine Receptor D3 (DRD3) (P35462).
Immunogen Sequence: MASLSQLSGHLNYTCGAENS TGASQARPHAYYALSYCALI LAIVFGNGLVCMAVLKERAL QTTTNYLVVSLAVADLLVAT LVMPWVVYLEVTGGVWNFSR
Tissue Specificity Brain.
Post Translational Modifications Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma). Palmitoylated.
Function Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
Protein Name D(3 Dopamine Receptor
Dopamine D3 Receptor
Database Links Reactome: R-HSA-390651
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Both Membrane-Bound And Scattered In The Cytoplasm During Basal Conditions
Receptor Stimulation Results In The Rapid Internalization And Sequestration Of The Receptors At The Perinuclear Area (5 And 15 Minutes)
Followed By The Dispersal Of The Receptors To The Membrane (30 Minutes)
Drd3 And Grk4 Co-Localize In Lipid Rafts Of Renal Proximal Tubule Cells
Alternative Antibody Names Anti-D(3 Dopamine Receptor antibody
Anti-Dopamine D3 Receptor antibody
Anti-DRD3 antibody

Information sourced from Uniprot.org

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