• Confocal imaging of MCF7 cells using DNPH1 Rabbit monoclonal antibody (STJ11103484, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using DNPH1 Rabbit monoclonal antibody (STJ11103484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DNPH1 antibody (75-174) [S4MR] (STJ11103484)

SKU:
STJ11103484

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNPH1
Gene ID: 10591
Uniprot ID: DNPH1_HUMAN
Immunogen Region: 75-174
Specificity: A synthetic peptide corresponding to a sequence within amino acids 75-174 of human DNPH1 (O43598).
Immunogen Sequence: LIHEQDLEWLQQADVVVAEV TQPSLGVGYELGRAVAFNKR ILCLFRPQSGRVLSAMIRGA ADGSRFQVWDYEEGEVEALL DRYFEADPPGQVAASPDPTT
Tissue Specificity Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.
Function Part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate). Catalyzes the second step in that pathway, the hydrolysis of the N-glycosidic bond in hmdUMP, degrading this cytotoxic nucleotide to avoid its genomic integration.
Protein Name 5-Hydroxymethyl-Dump N-Hydrolase
2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1
C-Myc-Responsive Protein Rcl
Database Links Reactome: R-HSA-74259
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-5-Hydroxymethyl-Dump N-Hydrolase antibody
Anti-2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1 antibody
Anti-C-Myc-Responsive Protein Rcl antibody
Anti-DNPH1 antibody
Anti-C6orf108 antibody
Anti-RCL antibody

Information sourced from Uniprot.org

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