| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNMT3B (345-853) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNMT3B |
| Gene ID: |
1789 |
| Uniprot ID: |
DNM3B_HUMAN |
| Immunogen Region: |
345-853 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 345-853 of human DNMT3B (NP_008823.1). |
| Immunogen Sequence: |
IEGLKPNNTQPVVNKSKVRR AGSRKLESRKYENKTRRRTA DDSATSDYCPAPKRLKTNCY NNGKDRGDEDQSREQMASDV ANNKSSLEDGCLSCGRKNPV SFHPLFEGGLCQTCRDRFLE LFYMYDDDGYQ |
| Tissue Specificity | Ubiquitous.highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. |
| Post Translational Modifications | Sumoylated. Citrullinated by PADI4. |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells. |
| Protein Name | DnaCytosine-5-Methyltransferase 3bDnmt3bDna Methyltransferase HsaiiibDna Mtase HsaiiibM.hsaiiib |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-427413Reactome: R-HSA-4655427Reactome: R-HSA-5334118Reactome: R-HSA-9710421 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3b antibodyAnti-Dnmt3b antibodyAnti-Dna Methyltransferase Hsaiiib antibodyAnti-Dna Mtase Hsaiiib antibodyAnti-M.hsaiiib antibodyAnti-DNMT3B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
