| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNAJC2 (1-140) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNAJC2 |
| Gene ID: |
27000 |
| Uniprot ID: |
DNJC2_HUMAN |
| Immunogen Region: |
1-140 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human DNAJC2 (NP_055192.1). |
| Immunogen Sequence: |
MLLLPSAADGRGTAITHALT SASTLCQVEPVGRWFEAFVK RRNRNASASFQELEDKKELS EESEDEELQLEEFPMLKTLD PKDWKNQDHYAVLGLGHVRY KATQRQIKAAHKAMVLKHHP DKRKAAGEPIKEGDNDYFTC |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated in M (mitotic) phase. |
| Function | Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. |
| Protein Name | Dnaj Homolog Subfamily C Member 2M-Phase Phosphoprotein 11Zuotin-Related Factor 1 Cleaved Into - Dnaj Homolog Subfamily C Member 2 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-3371453 |
| Cellular Localisation | NucleusCytoplasmCytosol |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily C Member 2 antibodyAnti-M-Phase Phosphoprotein 11 antibodyAnti-Zuotin-Related Factor 1 Cleaved Into - Dnaj Homolog Subfamily C Member 2 - N-Terminally Processed antibodyAnti-DNAJC2 antibodyAnti-MPHOSPH11 antibodyAnti-MPP11 antibodyAnti-ZRF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DNAJC2 knockout (KO) 293T cells, using [KO Validated] DNAJC2 Rabbit polyclonal antibody (STJ11100830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89766/358240/STJ11100830_1__88794.1713122573.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJC2 knockout (KO) 293T cells, using [KO Validated] DNAJC2 Rabbit polyclonal antibody (STJ11100830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
