Host: | Rabbit |
Applications: | WB/IP/ELISA |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:2000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Gene Symbol: | LIG3 |
Gene ID: | 3980 |
Uniprot ID: | DNLI3_HUMAN |
Immunogen Region: | 730-1009 aa |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human DNA Ligase III/LIG3 (NP_039269.2). |
Immunogen Sequence: | AGGHDDATLARLQNELDMVK ISKDPSKIPSWLKVNKIYYP DFIVPDPKKAAVWEITGAEF SKSEAHTADGISIRFPRCTR IRDDKDWKSATNLPQLKELY QLSKEKADFTVVAGDEGSST TGGSSEENKGPSGSAVSRKA PSKPSASTKKAEGKLSNSNS KDGNMQTAKPSAMKVGEKLA TKSSPVKVGEKRKAADETLC QTKVLLDIFTGVRLYLPPST PDFSRLRRYFVAFDGDLVQ |
Tissue Specificity | Testis, thymus, prostate and heart. |
Function | Isoform 3 functions as a heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as a DNA ligase in mitochondrial base-excision DNA repair. |
Protein Name | Dna Ligase 3 Dna Ligase Iii Polydeoxyribonucleotide Synthase Atp 3 |
Database Links | Reactome: R-HSA-110381 Reactome: R-HSA-5649702 Reactome: R-HSA-5685939 Reactome: R-HSA-5696397 Reactome: R-HSA-6782210 |
Cellular Localisation | Isoform 1: Mitochondrion Contains An N-Terminal Mitochondrial Transit Peptide Isoform 2: Mitochondrion Isoform 3: Nucleus Lacks The N-Terminal Mitochondrial Transit Peptide Isoform 4: Nucleus |
Alternative Antibody Names | Anti-Dna Ligase 3 antibody Anti-Dna Ligase Iii antibody Anti-Polydeoxyribonucleotide Synthase Atp 3 antibody Anti-LIG3 antibody |
Information sourced from Uniprot.org