| Applications: | WB/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LIG3 |
| Gene ID: | 3980 |
| Uniprot ID: | DNLI3_HUMAN |
| Immunogen Region: | 730-1009 aa |
| Immunogen Sequence: | AGGHDDATLARLQNELDMVK ISKDPSKIPSWLKVNKIYYP DFIVPDPKKAAVWEITGAEF SKSEAHTADGISIRFPRCTR IRDDKDWKSATNLPQLKELY QLSKEKADFTVVAGDEGSST TGGSSEENKGPSGSAVSRKA PSKPSASTKKAEGKLSNSNS KDGNMQTAKPSAMKVGEKLA TKSSPVKVGEKRKAADETLC QTKVLLDIFTGVRLYLPPST PDFSRLRRYFVAFDGDLVQ |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human DNA Ligase III/LIG3 (NP_039269.2). |
| Tissue Specificity | Testis, thymus, prostate and heart. |
| Function | Isoform 3 functions as a heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as a DNA ligase in mitochondrial base-excision DNA repair. |
| Protein Name | Dna Ligase 3Dna Ligase IiiPolydeoxyribonucleotide Synthase Atp 3 |
| Database Links | Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5685939Reactome: R-HSA-5696397Reactome: R-HSA-6782210Reactome: R-HSA-9913635 P49916-1 |
| Cellular Localisation | Isoform 1: MitochondrionContains An N-Terminal Mitochondrial Transit PeptideIsoform 2: MitochondrionIsoform 3: NucleusLacks The N-Terminal Mitochondrial Transit PeptideIsoform 4: Nucleus |
| Alternative Antibody Names | Anti-Dna Ligase 3 antibodyAnti-Dna Ligase Iii antibodyAnti-Polydeoxyribonucleotide Synthase Atp 3 antibodyAnti-LIG3 antibody |
Information sourced from Uniprot.org
