| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | LIG3 |
| Gene ID: | 3980 |
| Uniprot ID: | DNLI3_HUMAN |
| Immunogen Region: | 730-1009 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human DNA Ligase III/LIG3 (NP_039269.2). |
| Immunogen Sequence: | AGGHDDATLARLQNELDMVK ISKDPSKIPSWLKVNKIYYP DFIVPDPKKAAVWEITGAEF SKSEAHTADGISIRFPRCTR IRDDKDWKSATNLPQLKELY QLSKEKADFTVVAGDEGSST TGGSSEENKGPSGSAVSRKA PSKPSASTKKAEGKLSNSNS KDGNMQTAKPSAMKVGEKLA TKSSPVKVGEKRKAADETLC QTKVLLDIFTGVRLYLPPST PDFSRLRRYFVAFDGDLVQ |
| Tissue Specificity | Testis, thymus, prostate and heart. |
| Function | Isoform 3 functions as a heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as a DNA ligase in mitochondrial base-excision DNA repair. |
| Protein Name | Dna Ligase 3Dna Ligase IiiPolydeoxyribonucleotide Synthase Atp 3 |
| Database Links | Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5685939Reactome: R-HSA-5696397Reactome: R-HSA-6782210 |
| Cellular Localisation | Isoform 1: MitochondrionContains An N-Terminal Mitochondrial Transit PeptideIsoform 2: MitochondrionIsoform 3: NucleusLacks The N-Terminal Mitochondrial Transit PeptideIsoform 4: Nucleus |
| Alternative Antibody Names | Anti-Dna Ligase 3 antibodyAnti-Dna Ligase Iii antibodyAnti-Polydeoxyribonucleotide Synthase Atp 3 antibodyAnti-LIG3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit pAb (STJ11106799) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/185995/407755/STJ11106799_1__67131.1744894266.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit pAb (STJ11106799) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1min.")
