Western blot analysis of lysates from Jurkat cells, using DNA Ligase I Rabbit mAb (STJ11103656) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-DNA Ligase I antibody (1-91) [S6MR] (STJ11103656)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103656
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General Information

Short DescriptionRabbit monoclonal DNA Ligase I (1-91) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS6MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolLIG1
Gene ID3978
Uniprot IDDNLI1_HUMAN
Immunogen Region1-91
Immunogen SequenceMQRSIMSFFHPKKEGKAKKP EKEASNSSRETEPPPKAALK EWNGVVSESDSPVKRPGRKA ARVLGSEGEEEDEALSPAKG QKPALDCSQVS
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-91 of human DNA Ligase I (P18858).

Additional Info

Function DNA ligase that seals nicks in double-stranded during DNA repair. Also involved in DNA replication and DNA recombination.
Protein Name Dna Ligase 1
Dna Ligase I
Polydeoxyribonucleotide Synthase Atp 1
Database Links Reactome: R-HSA-110362
Reactome: R-HSA-162594
Reactome: R-HSA-174414
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Reactome: R-HSA-69183
Reactome: R-HSA-9825895
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Ligase 1 antibody
Anti-Dna Ligase I antibody
Anti-Polydeoxyribonucleotide Synthase Atp 1 antibody
Anti-LIG1 antibody

Information sourced from Uniprot.org

Citations

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