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Western blot analysis of extracts of Jurkat cells, using DNA Ligase I antibody (STJ11103656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Immunofluorescence analysis of U-2 OS cells using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat testis using DNA Ligase I rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human breast cancer using DNA Ligase I rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens).
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Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded human colon carcinoma tissue using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry of paraffin-embedded mouse lung using DNA Ligase I rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens).
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Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded mouse brain tissue using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunofluorescence analysis of C6 cells using DNA Ligase I rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded human thyroid cancer tissue using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunofluorescence analysis of U-2 OS cells using DNA Ligase I rabbit monoclonal antibody (STJ11103656) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded rat brain tissue using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded human spleen tissue using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Immunohistochemistry analysis of DNA Ligase I in paraffin-embedded rat lung tissue using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
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Western blot analysis of lysates from Jurkat cells, using DNA Ligase I Rabbit monoclonal antibody (STJ11103656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.