• Confocal imaging of paraffin-embedded Rat skeletal muscle tissue using Dystrophin Rabbit monoclonal antibody (STJ11105697, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using Dystrophin Rabbit monoclonal antibody (STJ11105697, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Confocal imaging of paraffin-embedded Human skeletal muscle tissue using Dystrophin Rabbit monoclonal antibody (STJ11105697, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
  • Immunohistochemistry analysis of Dystrophin in paraffin-embedded mouse heart using Dystrophin Rabbit monoclonal antibody (STJ11105697) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Dystrophin in paraffin-embedded rat skeletal muscle using Dystrophin Rabbit monoclonal antibody (STJ11105697) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DMD antibody (3586-3685) [S5697RM] (STJ11105697)

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STJ11105697

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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Dystrophin (3586-3685) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5697RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DMD
Gene ID: 1756
Uniprot ID: DMD_HUMAN
Immunogen Region: 3586-3685
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 3586-3685 of human Dystrophin (P11532).
Immunogen Sequence: LESQLHRLRQLLEQPQAEAK VNGTTVSSPSTSLQRSDSSQ PMLLRVVGSQTSDSMGEEDL LSPPQDTSTGLEEVMEQLNN SFPSSRGRNTPGKPMREDTM
Tissue Specificity Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Most tissues contain transcripts of multiple isoforms. Isoform 15: Only isoform to be detected in heart and liver and is also expressed in brain, testis and hepatoma cells.
Function Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
Protein Name Dystrophin
Database Links Reactome: R-HSA-3000171
Reactome: R-HSA-390522
Cellular Localisation Cell Membrane
Sarcolemma
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Postsynaptic Cell Membrane
In Muscle Cells
Sarcolemma Localization Requires The Presence Of Ank2
While Localization To Costameres Requires The Presence Of Ank3
Localizes To Neuromuscular Junctions (Nmjs)
In Adult Muscle
Nmj Localization Depends Upon Ank2 Presence
But Not In Newborn Animals
Alternative Antibody Names Anti-Dystrophin antibody
Anti-DMD antibody

Information sourced from Uniprot.org

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