Western blot analysis of extracts of various cell lines, using DLX5 Rabbit monoclonal antibody (STJ11102311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DLX5 antibody (150-250) [S1MR] (STJ11102311)

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STJ11102311
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLX5
Gene ID: 1749
Uniprot ID: DLX5_HUMAN
Immunogen Region: 150-250
Specificity: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DLX5 (P56178).
Immunogen Sequence: AALQRRFQKTQYLALPERAE LAASLGLTQTQVKIWFQNKR SKIKKIMKNGEMPPEHSPSS SDPMACNSPQSPAVWEPQGS SRSLSHHPHAHPPTSNQSPA S
Post Translational Modifications Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability.
Function Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.
Protein Name Homeobox Protein Dlx-5
Database Links Reactome: R-HSA-8939902
Reactome: R-HSA-9834899
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Dlx-5 antibody
Anti-DLX5 antibody

Information sourced from Uniprot.org

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