| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DLG1 |
| Gene ID: | 1739 |
| Uniprot ID: | DLG1_HUMAN |
| Immunogen Region: | 1-190 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2). |
| Immunogen Sequence: | MPVRKQDTQRALHLLEEYRS KLSQTEDRQLRSSIERVINI FQSNLFQALIDIQEFYEVTL LDNPKCIDRSKPSEPIQPVN TWEISSLPSSTVTSETLPSS LSPSVEKYRYQDEDTPPQEH ISPQITNEVIGPELVHVSEK NLSEIENVHGFVSHSHISPI KPTEAVLPSPPTVPVIPVLP VPAENTVILP |
| Tissue Specificity | Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Expressed in the brain (at protein level). Widely expressed, with isoforms displaying different expression profiles. |
| Post Translational Modifications | Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A. Ubiquitinated.by MARCHF2 which results in its degradation. |
| Function | Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion. May also play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane. |
| Protein Name | Disks Large Homolog 1 Synapse-Associated Protein 97 Sap-97 Sap97 Hdlg |
| Database Links | Reactome: R-HSA-399719 Reactome: R-HSA-438066 Reactome: R-HSA-442982 Reactome: R-HSA-447038 Reactome: R-HSA-451308 Reactome: R-HSA-5673001 Reactome: R-HSA-8849932 Reactome: R-HSA-9609736 Reactome: R-HSA-9617324 Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell Membrane Peripheral Membrane Protein Basolateral Cell Membrane Endoplasmic Reticulum Membrane Postsynaptic Density Synapse Sarcolemma Apical Cell Membrane Cell Junction Cytoplasm Colocalizes With Epb41 At Regions Of Intercellular Contacts Basolateral In Epithelial Cells May Also Associate With Endoplasmic Reticulum Membranes Mainly Found In Neurons Soma Moderately Found At Postsynaptic Densities |
| Alternative Antibody Names | Anti-Disks Large Homolog 1 antibody Anti-Synapse-Associated Protein 97 antibody Anti-Sap-97 antibody Anti-Sap97 antibody Anti-Hdlg antibody Anti-DLG1 antibody |
Information sourced from Uniprot.org