| Host: |
Rabbit |
| Applications: |
WB/IHC-F/IHC-P/ICC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Lipoamide Dehydrogenase (488-500 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
R05-6D3 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: |
Affinity Purified |
| Concentration: |
0.3 mg/ml |
| Dilution Range: |
WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Storage Instruction: |
Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: |
DLD |
| Gene ID: |
1738 |
| Uniprot ID: |
DLDH_HUMAN |
| Immunogen Region: |
488-500 aa |
| Immunogen: |
A synthetic peptide of human Lipoamide Dehydrogenase |
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
| Protein Name | Dihydrolipoyl Dehydrogenase - MitochondrialDihydrolipoamide DehydrogenaseGlycine Cleavage System L Protein |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-6783984Reactome: R-HSA-70895Reactome: R-HSA-9837999Reactome: R-HSA-9853506Reactome: R-HSA-9858328Reactome: R-HSA-9859138Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion MatrixNucleusCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-Dihydrolipoyl Dehydrogenase - Mitochondrial antibodyAnti-Dihydrolipoamide Dehydrogenase antibodyAnti-Glycine Cleavage System L Protein antibodyAnti-DLD antibodyAnti-GCSL antibodyAnti-LAD antibodyAnti-PHE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
