| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-DKK4 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30-35kDa band seen in Jurkat lysate. Recommended for use at 1-3µg/ml. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DKK4 |
| Gene ID: | 27121 |
| Uniprot ID: | DKK4_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_055235.1 |
| Immunogen Sequence: | RQHARLRVCQKIEKL |
| Post Translational Modifications | Appears to be not glycosylated. Can be proteolytically processed by a furin-like protease. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
| Protein Name | Dickkopf-Related Protein 4Dickkopf-4Dkk-4Hdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3772470Reactome: R-HSA-5339717 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Dickkopf-Related Protein 4 antibodyAnti-Dickkopf-4 antibodyAnti-Dkk-4 antibodyAnti-Hdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form antibodyAnti-DKK4 antibody |
Information sourced from Uniprot.org