Anti-DKK4 antibody (C-Term) (STJ70138)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70138-100
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General Information

Short DescriptionGoat polyclonal antibody anti-DKK4 (C-Term) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Pig
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 30-35kDa band seen in Jurkat lysate. Recommended for use at 1-3µg/ml. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolDKK4
Gene ID27121
Uniprot IDDKK4_HUMAN
Accession NumberNP_055235.1
Immunogen RegionC-Term
Immunogen SequenceRQHARLRVCQKIEKL

Additional Info

Post Translational Modifications Appears to be not glycosylated. Can be proteolytically processed by a furin-like protease.
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Protein Name Dickkopf-Related Protein 4
Dickkopf-4
Dkk-4
Hdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Reactome: R-HSA-5339717
Cellular Localisation Secreted
Alternative Antibody Names Anti-Dickkopf-Related Protein 4 antibody
Anti-Dickkopf-4 antibody
Anti-Dkk-4 antibody
Anti-Hdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form antibody
Anti-DKK4 antibody

Information sourced from Uniprot.org

Citations

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