• Western blot analysis of extracts of various cell lines, using DKK3 antibody (STJ11102858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of mouse heart, using DKK3 antibody (STJ11102858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of C6 cells using DKK3 rabbit polyclonal antibody (STJ11102858) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using DKK3 rabbit polyclonal antibody (STJ11102858) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using DKK3 rabbit polyclonal antibody (STJ11102858) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DKK3 antibody (22-200) (STJ11102858)

SKU:
STJ11102858

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DKK3
Gene ID: 27122
Uniprot ID: DKK3_HUMAN
Immunogen Region: 22-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 22-200 of human DKK3 (NP_056965.3).
Immunogen Sequence: APAPTATSAPVKPGPALSYP QEEATLNEMFREVEELMEDT QHKLRSAVEEMEAEEAAAKA SSEVNLANLPPSYHNETNTD TKVGNNTIHVHREIHKITNN QTGQMVFSETVITSVGDEEG RRSHECIIDEDCGPSMYCQF ASFQYTCQPCRGQRMLCTRD SECCGDQLCVWGHCTKMAT
Tissue Specificity Highest expression in heart, brain, and spinal cord.
Post Translational Modifications N- and O-glycosylated.
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Protein Name Dickkopf-Related Protein 3
Dickkopf-3
Dkk-3
Hdkk-3
Cellular Localisation Secreted
Alternative Antibody Names Anti-Dickkopf-Related Protein 3 antibody
Anti-Dickkopf-3 antibody
Anti-Dkk-3 antibody
Anti-Hdkk-3 antibody
Anti-DKK3 antibody
Anti-REIC antibody
Anti-UNQ258 antibody
Anti-PRO295 antibody

Information sourced from Uniprot.org

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