Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-DIO2 antibody (STJ192992)

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STJ192992
£49.00 - £261.00
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rabbit polyclonal anti-Type II iodothyronine deiodinase for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DIO2
Gene ID: 1734
Uniprot ID: IOD2_HUMAN
Specificity: IOD2 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Post Translational Modifications Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.
Function Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (3,3'-T2) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD). Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine and 3',5'- diiodothyronamine.
Protein Name Type Ii Iodothyronine Deiodinase
5dii
Dioii
Type 2 Di
Type-Ii 5'-Deiodinase
Database Links Reactome: R-HSA-350864
Reactome: R-HSA-9844594
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iii Membrane Protein
Alternative Antibody Names Anti-Type Ii Iodothyronine Deiodinase antibody
Anti-5dii antibody
Anti-Dioii antibody
Anti-Type 2 Di antibody
Anti-Type-Ii 5'-Deiodinase antibody
Anti-DIO2 antibody
Anti-ITDI2 antibody
Anti-TXDI2 antibody

Information sourced from Uniprot.org

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