• Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 3 Mu g DHX36 antibody (STJ23377). Western blot was performed from the immunoprecipitate using DHX36 antibody (STJ23377) at a dilution of 1:1000.
  • Western blot analysis of various lysates using DHX36 Rabbit polyclonal antibody (STJ23377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DHX36 antibody (1-260) (STJ23377)

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STJ23377

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DHX36 (1-260) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DHX36
Gene ID: 170506
Uniprot ID: DHX36_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DHX36 (NP_065916.2).
Immunogen Sequence: MSYDYHQNWGRDGGPRSSGG GYGGGPAGGHGGNRGSGGGG GGGGGGRGGRGRHPGHLKGR EIGMWYAKKQGQKNKEAERQ ERAVVHMDERREEQIVQLLN SVQAKNDKESEAQISWFAPE DHGYGTEVSTKNTPCSENKL DIQEKKLINQEKKMFRIRNR SYIDRDSEYLLQENEPDGTL DQKLLEDLQKKKNDLRYIEM QHFREKLPSYGMQKELVNLI DNHQVTVISGETGCGKTTQ
Tissue Specificity Highly expressed in testis.
Function Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures. Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses. G4 structures correspond to helical structures containing guanine tetrads. Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-ADN and G4-RNA). Plays a role in genomic integrity. Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription. Plays a role in transcriptional regulation. Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression. Plays a role in post-transcriptional regulation. Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage. Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment. Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size. Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability. Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3'-UTR of the PITX1 mRNA regulating negatively PITX1 protein expression. Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3'-UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively. Binds also to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation. Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Required for early embryonic development and hematopoiesis. Involved in the regulation of cardioblast differentiation and proliferation during heart development. Involved in spermatogonia differentiation. May play a role in ossification.
Protein Name Atp-Dependent Dna/Rna Helicase Dhx36
Dead/H Box Polypeptide 36
Deah-Box Protein 36
G4-Resolvase-1
G4r1
Mle-Like Protein 1
Rna Helicase Associated With Au-Rich Element Protein
Database Links Reactome: R-HSA-3134963
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Stress Granule
Nucleus Speckle
Chromosome
Telomere
Mitochondrion
Perikaryon
Cell Projection
Dendrite
Axon
Predominantly Localized In The Nucleus
Colocalizes With Srsf2 In Nuclear Speckles
Colocalizes With Ddx5 In Nucleolar Caps Upon Transcription Inhibition
Accumulates And Colocalized With Tia1 In Cytoplasmic Stress Granules (Sgs) In An Arsenite-
Heat Shock- And Rna-Binding-Dependent Manner
Shuttles Into And Out Of Sgs In An Atpase-Dependent Manner
Colocalizes In The Cytosol With The Multi-Helicase-Ticam1 Complex That Translocates To The Mitochondria Upon Poly(I:c) Rna Ligand Stimulation
Isoform 1: Nucleus
Preferentially Localized In The Nucleus
Excluded From Nucleoli
Isoform 2: Nucleus
Preferentially Localized In The Cytoplasm
Alternative Antibody Names Anti-Atp-Dependent Dna/Rna Helicase Dhx36 antibody
Anti-Dead/H Box Polypeptide 36 antibody
Anti-Deah-Box Protein 36 antibody
Anti-G4-Resolvase-1 antibody
Anti-G4r1 antibody
Anti-Mle-Like Protein 1 antibody
Anti-Rna Helicase Associated With Au-Rich Element Protein antibody
Anti-DHX36 antibody
Anti-DDX36 antibody
Anti-KIAA1488 antibody
Anti-MLEL1 antibody
Anti-RHAU antibody

Information sourced from Uniprot.org

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