Anti-DGKI antibody (991-1040 aa) (STJ92703)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DGKI at the amino acid range 991-1040
General Information
| Short Description | Rabbit polyclonal anti-Diacylglycerol kinase iota (991-1040 aa) for use in WB, IHC, IF and ELISA in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DGKI |
| Gene ID | 9162 |
| Uniprot ID | DGKI_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DGKI at the amino acid range 991-1040 |
| Immunogen Region | 991-1040 aa |
| Specificity | DGK-Iota Polyclonal Antibody detects endogenous levels of DGK-Iota protein. |
Additional Info
| Function | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position. By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway. May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression. |
| Protein Name | Diacylglycerol Kinase IotaDag Kinase IotaDgk-Iota |
| Database Links | Reactome: R-HSA-114508 |
| Cellular Localisation | Cell ProjectionAxonDendritePresynapsePostsynapsePostsynaptic DensitySynaptic Cell MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneCytoplasmCytosolNucleusExcluded From Inhibitory SynapsesLocalization Between Cytoplasm And Nucleus Is Regulated By Protein Kinase CBoth In The Detergent Soluble And Particulate Fractions |
| Alternative Antibody Names | Anti-Diacylglycerol Kinase Iota antibodyAnti-Dag Kinase Iota antibodyAnti-Dgk-Iota antibodyAnti-DGKI antibody |
Information sourced from Uniprot.org