Anti-DES antibody (421-470 aa) (STJ92691)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Desmin at the amino acid range 421-470
General Information
| Short Description | Rabbit polyclonal anti-Desmin (421-470 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DES |
| Gene ID | 1674 |
| Uniprot ID | DESM_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Desmin at the amino acid range 421-470 |
| Immunogen Region | 421-470 aa |
| Specificity | Desmin Polyclonal Antibody detects endogenous levels of Desmin protein. |
Additional Info
| Function | Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin. |
| Protein Name | Desmin |
| Database Links | Reactome: R-HSA-390522 |
| Cellular Localisation | CytoplasmMyofibrilSarcomereZ LineCell MembraneSarcolemmaNucleusCell TipNucleus EnvelopeLocalizes In The Intercalated Disks Which Occur At The Z Line Of CardiomyocytesLocalizes In The Nucleus Exclusively In Differentiating Cardiac Progenitor Cells And Premature CardiomyocytesPkp2 Is Required For Correct Anchoring Of Des At The Cell Tip And Nuclear Envelope |
| Alternative Antibody Names | Anti-Desmin antibodyAnti-DES antibody |
Information sourced from Uniprot.org