• C2C12 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Desmin (ABT322) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: C2C12Predicted band size: 53kDaObserved band size: 53kDa
  • mouse muscle whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Desmin (ABT322) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: mouse musclePredicted band size: 53kDaObserved band size: 60kDa
  • Human appendix tissue was stained with Anti-Desmin (ABT322) Antibody
  • Human endometrial adenocarcinoma tissue was stained with Anti-Desmin (ABT322) Antibody
  • Human heart muscle tissue was stained with Anti-Desmin (ABT322) Antibody
  • Human prostate tissue was stained with Anti-Desmin (ABT322) Antibody
  • Human tonsil tissue was stained with Anti-Desmin (ABT322) Antibody

Anti-DES antibody (400-470 aa) [ABT322] (STJ197225)

SKU:
STJ197225

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Desmin (400-470 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT322
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DES
Gene ID: 1674
Uniprot ID: DESM_HUMAN
Immunogen Region: 400-470 aa
Specificity: The antibody can specifically recognize human Desmin protein.
Immunogen: Synthesized peptide derived from the human Desmin at the amino acid range 400-470
Function Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin.
Protein Name Desmin
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Z Line
Cell Membrane
Sarcolemma
Nucleus
Localizes In The Intercalated Disks Which Occur At The Z Line Of Cardiomyocytes
Localizes In The Nucleus Exclusively In Differentiating Cardiac Progenitor Cells And Premature Cardiomyocytes
Alternative Antibody Names Anti-Desmin antibody
Anti-DES antibody

Information sourced from Uniprot.org

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