Anti-DEPTOR antibody (1-217) (STJ113643)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DEPDC6 (1-217) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DEPTOR |
| Gene ID | 64798 |
| Uniprot ID | DPTOR_HUMAN |
| Immunogen Region | 1-217 |
| Immunogen Sequence | MEEGGSTGSAGSDSSTSGSG GAQQRELERMAEVLVTGEQL RLRLHEEKVIKDRRHHLKTY PNCFVAKELIDWLIEHKEAS DRETAIKLMQKLADRGIIHH VCDEHKEFKDVKLFYRFRKD DGTFPLDNEVKAFMRGQRLY EKLMSPENTLLQPREEEGVK YERTFMASEFLDWLVQEGEA TTRKEAEQLCHRLMEHGIIQ HVSNKHPFVDSNLLYQF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human DEPDC6 (NP_073620.2). |
Additional Info
| Post Translational Modifications | Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2. Phosphorylated at Ser-286, Ser-287 and Ser-291 in response to mitogenic stimulation by MTOR: DEPTOR is either directly phosphorylated by MTOR or indirectly via proteins kinases that are activated by MTOR, such as CK1/CSNK1A1. Phosphorylation at Ser-286, Ser-287 and Ser-291 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. Phosphorylation at Ser-235 by MAPK3/ERK1 promotes deubiquitination by USP7, enhancing its stability. Phosphorylation at Tyr-289 by SYK impairs its interaction with MTOR, promoting mTORC1 and mTORC2 signaling. Ubiquitinated.leading to proteasomal degradation. Ubiquitination by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-286, Ser-287 and Ser-291 by MTOR, leads to its degradation by the proteasome. Deubiquitinated by OTUB1 in response to amino acid via a non-canonical mechanism, leading to DEPTOR stability. Deubiquitinated by USP7 following phosphorylation at Ser-235, promoting its stability. |
| Function | Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity. |
| Protein Name | Dep Domain-Containing Mtor-Interacting ProteinHdeptorDep Domain-Containing Protein 6 |
| Database Links | |
| Cellular Localisation | Lysosome MembraneLocalizes To The Lysosomal Membrane When Associated With The Mtorc1 And Mtorc2 Complexes |
| Alternative Antibody Names | Anti-Dep Domain-Containing Mtor-Interacting Protein antibodyAnti-Hdeptor antibodyAnti-Dep Domain-Containing Protein 6 antibodyAnti-DEPTOR antibodyAnti-DEPDC6 antibody |
Information sourced from Uniprot.org