• Western blot analysis of extracts of various cell lines, using DEPDC6 rabbit polyclonal antibody (STJ113643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using DEPDC6 Rabbit polyclonal antibody (STJ113643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DEPTOR antibody (1-217) (STJ113643)

SKU:
STJ113643

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DEPDC6 (1-217) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DEPTOR
Gene ID: 64798
Uniprot ID: DPTOR_HUMAN
Immunogen Region: 1-217
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human DEPDC6 (NP_073620.2).
Immunogen Sequence: MEEGGSTGSAGSDSSTSGSG GAQQRELERMAEVLVTGEQL RLRLHEEKVIKDRRHHLKTY PNCFVAKELIDWLIEHKEAS DRETAIKLMQKLADRGIIHH VCDEHKEFKDVKLFYRFRKD DGTFPLDNEVKAFMRGQRLY EKLMSPENTLLQPREEEGVK YERTFMASEFLDWLVQEGEA TTRKEAEQLCHRLMEHGIIQ HVSNKHPFVDSNLLYQF
Post Translational Modifications Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2. Phosphorylated at Ser-286, Ser-287 and Ser-291 in response to mitogenic stimulation by MTOR: DEPTOR is either directly phosphorylated by MTOR or indirectly via proteins kinases that are activated by MTOR, such as CK1/CSNK1A1. Phosphorylation at Ser-286, Ser-287 and Ser-291 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. Phosphorylation at Ser-235 by MAPK3/ERK1 promotes deubiquitination by USP7, enhancing its stability. Phosphorylation at Tyr-289 by SYK impairs its interaction with MTOR, promoting mTORC1 and mTORC2 signaling. Ubiquitinated.leading to proteasomal degradation. Ubiquitination by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-286, Ser-287 and Ser-291 by MTOR, leads to its degradation by the proteasome. Deubiquitinated by OTUB1 in response to amino acid via a non-canonical mechanism, leading to DEPTOR stability. Deubiquitinated by USP7 following phosphorylation at Ser-235, promoting its stability.
Function Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity.
Protein Name Dep Domain-Containing Mtor-Interacting Protein
Hdeptor
Dep Domain-Containing Protein 6
Cellular Localisation Lysosome Membrane
Localizes To The Lysosomal Membrane When Associated With The Mtorc1 And Mtorc2 Complexes
Alternative Antibody Names Anti-Dep Domain-Containing Mtor-Interacting Protein antibody
Anti-Hdeptor antibody
Anti-Dep Domain-Containing Protein 6 antibody
Anti-DEPTOR antibody
Anti-DEPDC6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance