• Western blot analysis of Mouse lung, using Dectin-1/CLEC7A/CD369 Rabbit polyclonal antibody (STJ11105191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Dectin-1/CLEC7A/CD369 antibody (STJ11105191)

SKU:
STJ11105191

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dectin-1/CLEC7A/CD369 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Clec7aUniprot ID=CLC7A_MOUSE"
Immunogen: Recombinant protein of mouse Dectin-1/CLEC7A/CD369.
Tissue Specificity Detected in spleen (at protein level). Highly expressed in dendritic cells, spleen and thymus. Detected in epidermal Langerhans cells. Detected in macrophages, liver and lung.
Post Translational Modifications N-glycosylated. Phosphorylated on tyrosine residues in response to beta-glucan binding.
Function Lectin that functions as pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans.
Protein Name C-Type Lectin Domain Family 7 Member A
Beta-Glucan Receptor
C-Type Lectin Superfamily Member 12
Dendritic Cell-Associated C-Type Lectin 1
Dc-Associated C-Type Lectin 1
Dectin-1
Cd Antigen Cd369
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Enriched On Zymosan Phagosomes
Enrichment Does Not Depend On Opsonization
Alternative Antibody Names Anti-C-Type Lectin Domain Family 7 Member A antibody
Anti-Beta-Glucan Receptor antibody
Anti-C-Type Lectin Superfamily Member 12 antibody
Anti-Dendritic Cell-Associated C-Type Lectin 1 antibody
Anti-Dc-Associated C-Type Lectin 1 antibody
Anti-Dectin-1 antibody
Anti-Cd Antigen Cd369 antibody
Anti-Clec7a antibody
Anti-Bgr antibody
Anti-Clecsf12 antibody
Anti-Dectin1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance