• Western blot analysis of extracts of various cell lines, using DDX39A antibody (STJ114432) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-DDX39A antibody (STJ114432)

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against DDX39A.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: DX39A_HUMAN
Gene ID: 10212
Gene Symbol: DDX39A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human DDX39A (NP_005795.2).
Background This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Tissue Specificity Detected in testis, and at lower levels in brain, kidney, lung, thymus, spleen and salivary gland.
Database Links Reactome: R-HSA-159236
Cellular Localisation Nucleus
Can Translocate To The Cytoplasm In The Presence Of Mx1
Protein Name Atp-Dependent Rna Helicase Ddx39a
Dead Box Protein 39
Nuclear Rna Helicase Urh49
Alternative Names Anti-Atp-Dependent Rna Helicase Ddx39a antibody
Anti-Dead Box Protein 39 antibody
Anti-Nuclear Rna Helicase Urh49 antibody
Anti-DDX39A antibody
Anti-DDX39 antibody

Information sourced from uniprot.org

6 months standard