• Immunofluorescence analysis of L929 cells using DDX31 Rabbit polyclonal antibody (STJ118337) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using DDX31 Rabbit polyclonal antibody (STJ118337) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from A-549 cells, using DDX31 Rabbit polyclonal antibody (STJ118337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-DDX31 antibody (652-851) (STJ118337)

SKU:
STJ118337

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX31
Gene ID: 64794
Uniprot ID: DDX31_HUMAN
Immunogen Region: 652-851
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 652-851 of human DDX31 (NP_073616.6).
Immunogen Sequence: EIKMEDILCVLTRDDCFKGK RWGAQKSHAVGPQEIRERAT VLQTVFEDYVHSSERRVSWA KKALQSFIQAYATYPRELKH IFHVRSLHLGHVAKSFGLRD APRNLSALTRKKRKAHVKRP DLHKKTQSKHSLAEILRSEY SSGMEADIAKVKKQNAPGEP GGRPLQHSLQPTPCFGRGKT LKWRKTQKGVQRDSKTSQKV
Tissue Specificity Weakly or undetectably expressed in normal organs. Up-regulated in renal cell carcinoma.
Function May have DNA helicase activity and RNA helicase activity. Probably have ssDNA and RNA dependent ATPase activity. Plays a role in ribosome biogenesis and TP53/p53 regulation through its interaction with NPM1.
Protein Name Atp-Dependent Dna Helicase Ddx31
Dead Box Protein 31
Helicain
Probable Atp-Dependent Rna Helicase Ddx31
Cellular Localisation Nucleus
Nucleolus
Colocalized With Npm1 In The Nucleoli
Alternative Antibody Names Anti-Atp-Dependent Dna Helicase Ddx31 antibody
Anti-Dead Box Protein 31 antibody
Anti-Helicain antibody
Anti-Probable Atp-Dependent Rna Helicase Ddx31 antibody
Anti-DDX31 antibody

Information sourced from Uniprot.org

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